Regulates commercial animal feeds,
including pet and specialty pet food
manufactured or distributed in Indiana

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Mycotoxins in Feed Grains


The Office of Indiana State Chemist (OISC) tests feeds and ingredients for mycotoxins. Testing is performed for aflatoxin, fumonisin, zearalenone, T-2 toxin and vomitoxin or DON. Animals differ in their reaction to these toxins. FDA has established the following guidelines according to livestock specie as to the maximum toxin level that can be safely fed.

Class of Animal Feed Maximum
Aflatoxin Level
Immature animals and poultry Corn & peanut products 20 ppb
Dairy animals Corn & peanut products 20 ppb
Breeding cattle and swine Corn & peanut products 100 ppb
Mature poultry Corn & peanut products 100 ppb
Finishing swine over 100 lbs. Corn & peanut products 200 ppb
Finishing beef cattle Corn & peanut products 300 ppb
Beef cattle, swine or poultry Cottonseed Meal 300 ppb
All animals Other Feeds 20 ppb

Fumonisin - Fumonisin is a recently identified mycotoxin. Little published research is available relative to the safety of fumonisin. Fumonisin is known to cause equine leukoencephalomalcia in horses and pulmonary edema in pigs that eat contaminated corn. The Mycotoxin Committee of the American Association of Veterinary Laboratory Diagnosticians suggested in 1993 the following guidelines for fumonisin:


Class of Animal

Portion of Diet

Maximum Fumonisin Level

Horses Non-roughage portion of diet
**Do not feed corn screenings**
5 ppm
Swine Total ration 10 ppm
Poultry Total ration 50 ppm
Beef cattle Non-roughage portion of ration 50 ppm
Dairy cattle   No recommendation

NOTE: Fumonisin can be extremely hazardous to horses. Corn screenings should never be fed to horses. When present in corn or other grains, fumonisin can result in moldy corn poisoning or leukoencephalmalcia. Common symptoms are horses become disoriented, walk aimlessly, deranged behavior, colic, press head against solid objects, blindness, and death. Horses receiving a diet containing 5 ppm fumonisin are at risk. Symptoms will develop in 7 to 35 days.  Confirmation of death resulting from equine leukoencephalmalcia requires examination of the horse's brain for pathological features including softening and liquidifying of brain tissue. Prevalence of fumonisin will vary from year to year depending on weather conditions during the critical period of corn silking and ear development. Corn variety, insect damage and excess moisture during storage are also factors. A problem can be local or involve a specific storage bin or grower. Feed manufacturers selling corn or horse feed containing corn should consider conducting sufficient testing to ensure these feeds are safe for horses. The presence of fumonisin should alert feed manufacturers to monitor ingredients, especially corn, and evaluate the level of corn in horse feeds.

Vomitoxin - A toxin produced by several species of pink mold fusarium.  Toxin is also known as deoxynivalenol or DON.  It is a natural toxin that forms when conditions are cold and wet where the grain is grown.   In animals, especially swine, it causes vomiting, feed refusal, immune suppression, diarrhea, weight loss, and reduced milk production.  Wheat is common grain affected.   Toxin may concentrate in wheat by-products.  In 1995, a dog food manufacturer recalled 16,000 tons of product ($20 million loss) as a result of wheat by-product that contained >30 ppm vomitoxin.  FDA has established the following guidelines:


Class of Animal

Portion of Diet

Maximum Vomitoxin Level

Ruminating beef & feedlot cattle older than 4 months Grain & grain by-products not to exceed 50% of the diet 10 ppm
Chickens Grain & grain by-products not to exceed 50% of the diet 10 ppm
Swine Grain & grain by-products not to exceed 20% of the diet 5 ppm
All other animals Grain & grain by-products not to exceed 40% of the diet 5 ppm

Zearalenone - Zearalenone is another mycotoxin routinely checked by the Office of Indiana State Chemist. The critical level, that is the level considered to be unsatisfactory, is 0.5 ppm. The literature reports the effects and levels as follows; however, I haven't heard these levels as being suggested guidelines by FDA. They are, at least in the interim, something to use as a guide:

0.5 ppm may effect reproductive efficacy in young gilts 1.0 ppm may induce animal feminization 50-100 ppm can interfere with conception, ovulation, implantation, fetal development, and viability of newborn animals. 

T-2 Toxin - There is not much known about this one, however, when asked, we consider the critical level to be 0.5 ppm, based upon some North Dakota work.

At the present time in Indiana, we will use the levels cited here to determine whether an assay result is to be reported as "Satisfactory" or "Unsatisfactory" under the Indiana Commercial Feed Law (IC15-5-13).